A Review of Fine Root Dynamics in Populus Plantations

Production of native and hybridized varieties of Populus has received considerable interest in temperate regions as an alternative to agricultural crops and an additional wood source, while acting as a potential carbon (C) sink to offset emissions of fossil fuel-based greenhouse gases. Research of root system dynamics in Populus species is expanding, however, our understanding of the nature and role of fine roots (FR) is incomplete. The study objective, therefore, was to review the literature regarding FR production, mortality and longevity in Populus, and evaluate the magnitude and significance of the FR fraction to C sequestration. FRs, conventionally defined as less than 2 mm in diameter and responsible for water and nutrient uptake, are an essential component of the tree. Populus FRs are relatively short-lived, with reported lifespans ranging from 30 to 300 days, depending on root diameter, tree species and age, and soil environmental factors. Standing FR biomass fluctuates throughout the growing season. Fine root production generally peaks in mid-summer, and ranges between 1.0 and 5.0 mg ha⁻¹ yr⁻¹, while FR mortality has less seasonal amplitude. Production and mortality dynamics in Populus are highly plastic in response to soil environmental conditions, and although opposing conclusions have been proposed, research suggests soil moisture and nitrogen to be most important. Results from the literature indicate annual FR turnover to the soil C pool may be small (0.2–1.6 mg C ha⁻¹ yr⁻¹), but substantial in maintaining or enhancing C levels in natural and managed stands of Populus.     

发酵污泥不同施用方式对土壤温度和棉花苗期生理特性的影响

[目的]研究城市发酵污泥在棉花上的最佳施用方式,降低棉花生产成本。[方法]采用盆栽试验方法,探讨不施用发酵污泥(CK)、污泥覆盖土壤表面(处理(1))、污泥与土壤混合(处理(2))、混合+覆盖(处理(3))4种方式对棉苗、根系生长的影响。[结果]污泥的不同处理对棉花苗期根际土壤温度增温效果明显,以10 cm处土层最明显,处理(1)、(2)、(3)地温平均较对照CK高1.2、2.9、1.5℃;显著促进棉花苗的生长,棉花出苗率、健苗率、株高、叶面积、叶片数与CK相比极显著增加;对棉花苗根有诱导和促进生长作用,根长、根数量、根粗、根体积显著高于CK。[结论]发酵污泥3种不同施用方式都能够促进棉花苗生长,改善叶片和根的各项生理指标。整体来看,处理(2)综合优势好,是发酵污泥较有效的施用方式。     

Growth and weed suppression ability of common and new cover crops in Germany

Cover crops have a wide-ranging influence on the agroecosystem and create multiple benefits for farmers. A major benefit of cover crops is the suppression of weeds during fall and winter, which can help to reduce soil tillage and herbicide use. However, only a small number of cover crop species are currently grown in Germany. To enlarge this number, four new cover crop species including tartary buckwheat, forage radish, red oat and grain amaranth were tested in comparison with common cover crop species such as white mustard, oilseed radish and phacelia. Emergence, soil coverage, dry matter production and weed suppression ability was assessed for all cover crop species. White mustard emerged faster than all other cover crops and produced the highest amount of shoot dry matter at both locations in southwest Germany twelve weeks after planting (WAP). Oilseed radish was the only cover crop that reduced the weed dry matter in all experiments eight WAP. Phacelia was able to reduce weed density by 77% at Meiereihof twelve WAP. Tartary buckwheat offered the highest soil coverage four WAP, produced the greatest shoot dry matter eight WAP and reduced weed dry matter by more than 96% at Meiereihof and Ihinger Hof twelve WAP. Forage radish produced the highest root dry matter and reduced spring weed density by more than 81% in all experiments. Red oat and grain amaranth emerged slowly, produced less biomass than other cover crops and did not suppress weed growth. The results show that tartary buckwheat and forage radish are well suited as new cover crops in Germany due to their fast growth and good weed suppression ability.     

不同种植模式对土壤质量及马铃薯生长的影响

为探究轮作藜麦、玉米及连作对马铃薯根系生理及根系发育的影响及其机制,比较了3种种植模式(轮作藜麦、轮作玉米及连作)对马铃薯根际土壤微环境、根系生理、根系发育及植株生长的影响,以期为减轻马铃薯连作障碍、筛选较好的轮作模式提供理论依据。结果表明:(1)轮作藜麦、玉米明显降低土壤pH,提高土壤中有机质、碱解氮和有效磷含量,增强土壤肥力相关酶的活性,增加土壤细菌、放线菌数量和细菌与真菌数量比值(B/F),降低真菌数量,改善马铃薯根际土壤微环境,对植株生长发育起到促进作用,表现在马铃薯的株高、茎粗、地上部干重、根干重、单株薯重均有一定程度的增加。(2)轮作藜麦、玉米使得马铃薯根系超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性上升,超氧阴离子产生速率下降,丙二醛(MDA)含量减少,渗透调节物质含量增加,表明通过轮作藜麦和玉米使得连作对马铃薯植株造成的胁迫得到了一定程度缓解。(3)轮作藜麦、玉米显著提高了马铃薯根系总根长、根表面积、根体积、根平均直径和根尖数,说明轮作藜麦及玉米促进了马铃薯根系的生长发育,这与轮作藜麦及玉米改善土壤理化性质、生物学性质及促进马铃薯地上部分的发育相对应。比较轮作藜麦及轮作玉米的整体表现,以轮作玉米调控马铃薯连作障碍的效果较好。     

不同供磷水平对辣椒根系形态和根际特征的影响

以辣椒品种线椒王为试验材料，采用盆栽方式，通过分析不同施磷水平下辣椒根系形态和根系生理的变化，探究辣 椒磷高效利用的主要根系及根际过程。结果表明，辣椒地上部和根系生物量随供磷水平的增加呈现先增加后下降的趋势，整 株磷浓度以及整株磷累积量呈增加趋势。随供磷水平增加，根系总根长呈先增加后下降趋势，根系平均直径、根冠比呈逐渐 降低趋势；辣椒根系菌根侵染率和土壤酸性磷酸酶活性呈现先增加后下降的趋势。相关分析结果表明，土壤有效磷浓度和整 株磷浓度与根系平均直径、菌根侵染率呈极显著负相关，与总根长可用一元二次方程拟合，表明辣椒根系适应低磷的根际特 征可能是通过增加根系生物量及总根长，同时降低根系平均直径来实现的。     

切根与不同形态氮素对板栗苗木根系构型及生长的影响

为探明不同调控技术对板栗根系构型的影响和对板栗生长发育的作用效果,以期为培育高质量板栗苗木提供科学依据,采用大田板栗苗木为对象进行切根(切根深度分别为12、8、4 cm、CK)及不同形态氮素(尿素、硝酸钠、CK)追肥试验。结果表明:苗高生长量最大的处理为X2N2,地径生长量最大的处理为X4N1,不同处理苗木地径生长量差异性不显著;在同样切根深度的处理下,施用尿素的苗木在根系总根长、总表面积、总体积上均大于施用硝酸钠的苗木,也即NH^+4较NO^-3对板栗苗木根系生长发育具有更好的促进效果;X1N1、X2N1、X2N3处理在不同径级侧根的根长、根表面积、根体积上分别达到最高,差异性显著(P<0.05)。综合考虑苗木根系指标和形态指标,X2N1即切根深度8 cm结合施用尿素为板栗苗木根系的最佳调控处理。     

柠条生长季平茬对根系贮藏营养物质的影响

为了研究不同月份平茬对柠条根系贮存常规性营养物质和可溶性性营养物质的影响。采用饲料监测方法测定常规营养物质,参考《植物生理学实验指导》和《植物生理生化实验原理和技术》方法测定可溶性营养物质。结果表明,随着平茬时间推迟,柠条根系蛋白质含量呈逐渐下降的趋势,淀粉与脂肪之间相互转化协调逆境变化维持生命或再生发挥作用;可溶性碳水化合物是柠条根系主要贮藏的营养物质,对柠条不同月份平茬逆境胁迫下反应比较敏感;可溶性糖类与可溶性淀粉的比例逐渐随着月份增加,有利于植株抗击冻害;萌动期、初霜期平茬对柠条根系营养物质影响较大。柠条生长季平茬建议在4—8月进行。     

糜子愈伤诱导及再分化最适条件研究

研究旨在应用组织培养技术探究愈伤诱导及再分化的最适条件,以期为糜子建立遗传转化体系奠定基础。采用5个糜子品种研究愈伤诱导及再分化的最适条件。利用植物激素2,4-二氯苯氧乙酸(2,4-D)诱导茎尖愈伤组织,筛选出发芽率高、愈伤组织诱导率高、染菌率低的品种,并对诱导愈伤最适激素浓度及配比进行了鉴定。结果显示,‘冀黍2号’出芽率高、愈伤组织诱导率高、染菌率低,适宜进行组织培养;2,4-D对‘冀黍2号’愈伤诱导效果最好,最适浓度为2.5 mg/L,诱导率达86.67%,淡黄色块状,结构紧密,质地较硬,继代培养后形成的胚性愈伤组织状态更好。再分化过程中,2.5 mg/L 2,4-D+3.5 mg/L TDZ时,出现明显的嫩芽。该试验获得‘冀黍2号’愈伤诱导及再分化的最适条件,可为糜子再生体系的构建提供参考。     

Short time effects of biological and inter-row subsoiling on yield of potatoes grown on a loamy sand,and on soil penetration resistance,root growth and nitrogen uptake

Soil compaction, especially subsoil compaction, in agricultural fields has increased due to widespread use of heavy machines and intensification of vehicular traffic. Subsoil compaction changes the relative distribution of roots between soil layers and may restrict root development to the upper part of the soil profile, limiting water and mineral availability. This study investigated the direct effects of inter-row subsoiling, biological subsoiling and a combination of these two methods on soil penetration resistance, root length density, nitrogen uptake and yield. In field experiments with potatoes in 2013 and 2014, inter-row subsoiling (subsoiler) and biological subsoiling (preceding crops) were studied as two potential methods to reduce soil penetration resistance. Inter-row subsoiling was carried out post planting and the preceding crops were established one year, or in one case two years, prior to planting. Soil resistance was determined with a penetrometer three weeks after the potatoes were planted and root length density was measured after soil core sampling 2 months after emergence. Nitrogen uptake was determined in haulm (at haulm killing) and tubers (at harvest). Inter-row subsoiling had the greatest effect on soil penetration resistance, whereas biological subsoiling showed no effects. Root length density (RDL) in the combined treatment was higher than in the separate inter-row and biological subsoiling treatments and the control, whereas for the separate inter-row and biological subsoiling treatments, RLD was higher than in the control. Nitrogen uptake increased with inter-row subsoiling and was significantly higher than in the biological subsoiling and control treatments. However, in these experiments with a good supply of nutrients and water, no yield differences between any treatments were observed.     

Greater humification of belowground than aboveground biomass carbon into particulate soil organic matter in no-till corn and soybean crops

Quantifying the amount of carbon (C) incorporated from decomposing residues into soil organic carbon (C_S) requires knowing the rate of C stabilization (humification rate) into different soil organic matter pools. However, the differential humification rates of C derived from belowground and aboveground biomass into C_S pools has been poorly quantified. We estimated the contribution of aboveground and belowground biomass to the formation of C_S in four agricultural treatments by measuring changes in δ¹³C natural abundance in particulate organic matter (C_POM) associated with manipulations of C₃ and C₄ biomass. The treatments were (1) continuous corn cropping (C₄ plant), (2) continuous soybean cropping (C₃), and two stubble exchange treatments (3 and 4) where the aboveground biomass left after the grain harvest was exchanged between corn and soybean plots, allowing the separation of aboveground and belowground C inputs to C_S based on the different δ¹³C signatures. After two growing seasons, C_POM was primarily derived from belowground C inputs, even though they represented only ∼10% of the total plant C inputs as residues. Belowground biomass contributed from 60% to almost 80% of the total new C present in the C_POM in the top 10 cm of soil. The humification rate of belowground C inputs into C_POM was 24% and 10%, while that of aboveground C inputs was only 0.5% and 1.0% for soybean and corn, respectively. Our results indicate that roots can play a disproportionately important role in the C_POM budget in soils. Keywords Particulate organic matter; root carbon inputs; carbon isotopes; humification rate; corn; soybean.